Description: Rabbit polyclonal antibody to DNA Polymerase beta Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human DNA Polymerase beta. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: POLB Alternative Names: DNA polymerase betaEntrez Gene (Human): 5423Entrez Gene (Mouse) : 18970SwissProt (Human): P06746SwissProt (Mouse) : Q8K409Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of DNA Polymerase beta expression in HeLa (A), NIH3T3 (B), rat brain (C) whole cell lysates.
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  Immunohistochemical analysis of DNA Polymerase beta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.