Description: Rabbit polyclonal antibody to Phospholipase D2 (Phospho-Y169) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y169 of human Phospholipase D2 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: PLD2 Alternative Names: Phospholipase D2; PLD 2; hPLD2; Choline phosphatase 2; PLD1C; Phosphatidylcholine-hydrolyzing phospholipase D2Entrez Gene (Human): 5338Entrez Gene (Mouse) : 18806Entrez Gene (Rat) : 25097SwissProt (Human): O14939SwissProt (Mouse) : P97813SwissProt (Rat) : P70498Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Phospholipase D2 (Phospho-Y169) expression in HEK293T TNFa-treated (A), NIH3T3 TNFa-treated (B), H9C2 TNFa-treated (C) whole cell lysates.
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  Immunohistochemical analysis of Phospholipase D2 (Phospho-Y169) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.