Description: Rabbit polyclonal antibody to PLC gamma 2 (Phospho-Y753) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y753 of human PLC gamma 2 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: PLCG2 Alternative Names: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2; Phosphoinositide phospholipase C-gamma-2; Phospholipase C-IV; PLC-IV; Phospholipase C-gamma-2; PLC-gamma-2Entrez Gene (Human): 5336Entrez Gene (Mouse) : 234779Entrez Gene (Rat) : 29337SwissProt (Human): P16885SwissProt (Mouse) : Q8CIH5SwissProt (Rat) : P24135Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PLC gamma 2 (Phospho-Y753) expression in Hela TNFa-treated (A), MCF7 (B), Raw264.7 TNFa-treated (C), mouse kidney (D), rat kidney (E) whole cell lysates.
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  Immunohistochemical analysis of PLC gamma 2 (Phospho-Y753) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.