Description: Rabbit polyclonal antibody to PLC gamma 1 (Phospho-Y1253) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y1253 of human PLC gamma 1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) Gene Symbol: PLCG1 Alternative Names: PLC1; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1; PLC-148; Phosphoinositide phospholipase C-gamma-1; Phospholipase C-II; PLC-II; Phospholipase C-gamma-1; PLC-gamma-1Entrez Gene (Human): 5335Entrez Gene (Mouse) : 18803Entrez Gene (Rat) : 25738SwissProt (Human): P19174SwissProt (Mouse) : Q62077SwissProt (Rat) : P10686Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PLC gamma 1 (Phospho-Y1253) expression in MCF7 EGF-treated (A), A431 (B), mouse brain (C), mouse liver (D), H9C2 EGF-treated (E) whole cell lysates.
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Immunofluorescent analysis of PLC gamma 1 (Phospho-Y1253) staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Molecular and functional characterizations of the association and interactions between nucleophosmin-anaplastic lymphoma kinase and type I insulin-like growth factor receptor