Description: Rabbit polyclonal antibody to PLC beta 3 (Phospho-S537) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S537 of human PLC beta 3 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: PLCB3 Alternative Names: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3; Phosphoinositide phospholipase C-beta-3; Phospholipase C-beta-3; PLC-beta-3Entrez Gene (Human): 5331SwissProt (Human): Q01970SwissProt (Mouse) : P51432SwissProt (Rat) : Q99JE6Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PLC beta 3 (Phospho-S537) expression in HeLa PMA-treated (A), MCF7 UV-treated (B), mouse brain (C), rat brain (D) whole cell lysates.
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Immunohistochemical analysis of PLC beta 3 (Phospho-S537) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.