Description: Rabbit polyclonal antibody to PFKFB2 (Phospho-S483) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S483 of human PFKFB2 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: PFKFB2 Alternative Names: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2; 6PF-2-K/Fru-2,6-P2ase 2; PFK/FBPase 2; 6PF-2-K/Fru-2,6-P2ase heart-type isozymeEntrez Gene (Human): 5208Entrez Gene (Mouse) : 18640Entrez Gene (Rat) : 24640SwissProt (Human): O60825SwissProt (Mouse) : P70265SwissProt (Rat) : Q9JJH5Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PFKFB2 (Phospho-S483) expression in HEK293T (A) whole cell lysates.
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Immunohistochemical analysis of PFKFB2 (Phospho-S483) staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.