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Anti-PDPK1 (Phospho-S241) Antibody CPA1870
  • Western blot analysis of PDPK1 (Phospho-S241) expression in mouse liver (A), mouse lung (B), rat liver (C) whole cell lysates.
  • Immunohistochemical analysis of PDPK1 (Phospho-S241) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product NameAnti-PDPK1 (Phospho-S241) Antibody
Cat No: CPA1870
Source: Rabbit
Reactivity: H, M, R, B, P, Rb, S
Applications: WB, IH
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
200 μl
$420
100 μl
$260
30 μl
$130
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Description: Rabbit polyclonal antibody to PDPK1 (Phospho-S241)
Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S241 of human PDPK1 protein. The exact sequence is proprietary.
Purification: The antibody was purified by immunogen affinity chromatography.
Clonality: Polyclonal
Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100)
Gene Symbol: PDPK1
Alternative Names: PDK1; 3-phosphoinositide-dependent protein kinase 1; hPDK1
Entrez Gene (Human): 5170
Entrez Gene (Mouse) : 18607
Entrez Gene (Rat) : 81745
SwissProt (Human): O15530
SwissProt (Mouse) : Q9Z2A0
SwissProt (Rat) : O55173
Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of PDPK1 (Phospho-S241) expression in mouse liver (A), mouse lung (B), rat liver (C) whole cell lysates.
  • Immunohistochemical analysis of PDPK1 (Phospho-S241) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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