Description: Rabbit polyclonal antibody to PDGFA Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PDGFA. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: PDGFA Alternative Names: PDGF1; Platelet-derived growth factor subunit A; PDGF subunit A; PDGF-1; Platelet-derived growth factor A chain; Platelet-derived growth factor alpha polypeptideEntrez Gene (Human): 5154Entrez Gene (Mouse) : 18590Entrez Gene (Rat) : 25266SwissProt (Human): P04085SwissProt (Mouse) : P20033SwissProt (Rat) : P28576Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PDGFA expression in mouse lung (A) whole cell lysates.
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Immunohistochemical analysis of PDGFA staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.