Anti-PCNA Antibody 
Datasheet
MSDS
Description: Rabbit polyclonal antibody to PCNA Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PCNA. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: PCNA Alternative Names: Proliferating cell nuclear antigen; PCNA; CyclinEntrez Gene (Human): 5111Entrez Gene (Mouse) : 18538Entrez Gene (Rat) : 25737SwissProt (Human): P12004SwissProt (Mouse) : P17918SwissProt (Rat) : P04961Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PCNA expression in H9C2 (A), 3T3L1 (B), HEK293T (C), A549 (D), HCT116 (E) whole cell lysates. -
Immunohistochemical analysis of PCNA staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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