Description: Rabbit polyclonal antibody to PAK3 (Phospho-S154) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S154 of human PAK3 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: PAK3 Alternative Names: OPHN3; Serine/threonine-protein kinase PAK 3; Beta-PAK; Oligophrenin-3; p21-activated kinase 3; PAK-3Entrez Gene (Human): 5063Entrez Gene (Mouse) : 18481Entrez Gene (Rat) : 29433SwissProt (Human): O75914SwissProt (Mouse) : Q61036SwissProt (Rat) : Q62829Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PAK3 (Phospho-S154) expression in BV2 (A), SKOVCAR3 (B), U87MG (C) whole cell lysates.
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  Immunohistochemical analysis of PAK3 (Phospho-S154) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.