Description: Rabbit polyclonal antibody to NOTCH2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NOTCH2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: NOTCH2 Alternative Names: Neurogenic locus notch homolog protein 2; Notch 2; hN2Entrez Gene (Human): 4853Entrez Gene (Rat) : 29492SwissProt (Human): Q04721SwissProt (Rat) : Q9QW30Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of NOTCH2 expression in HEK293T (A), HepG2 (B) whole cell lysates.
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Immunohistochemical analysis of NOTCH2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Skp1-Cullin-F-box (SCF)-type ubiquitin ligase FBXW7 negatively regulates spermatogonial stem cell self-renewal
Impaired JNK signaling cooperates with KrasG12D expression to accelerate pancreatic ductal adenocarcinoma
Notch signaling in osteocytes differentially regulates cancellous and cortical bone remodeling
Mutations in the NOTCH pathway regulator MIB1 cause left ventricular noncompaction cardiomyopathy
ERK inhibition enhances TSA-induced gastric cancer cell apoptosis via NF-?B-dependent and Notch-independent mechanism
Notch signaling regulates mouse and human th17 differentiation
Expression of Notch receptors, ligands, and target genes during development of the mouse mammary gland
Effects of liquiritigenin treatment on the learning and memory deficits induced by amyloid beta-peptide (25-35) in rats