Description: Rabbit polyclonal antibody to nNOS Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human nNOS. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: NOS1 Alternative Names: Nitric oxide synthase, brain; Constitutive NOS; NC-NOS; NOS type I; Neuronal NOS; N-NOS; nNOS; Peptidyl-cysteine S-nitrosylase NOS1; bNOSEntrez Gene (Human): 4842Entrez Gene (Mouse) : 18125Entrez Gene (Rat) : 24598SwissProt (Human): P29475SwissProt (Mouse) : Q9Z0J4SwissProt (Rat) : P29476Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of nNOS expression in HeLa (A), Raw264.7 (B), mouse brain (C), PC12 (D), rat brain (E) whole cell lysates.
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  Immunohistochemical analysis of nNOS staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.