Description: Rabbit polyclonal antibody to IKB alpha (Phospho-S32/S36) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S32/S36 of human IKB alpha protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500) Gene Symbol: NFKBIA Alternative Names: IKBA; MAD3; NFKBI; NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3Entrez Gene (Human): 4792Entrez Gene (Mouse) : 18035Entrez Gene (Rat) : 25493SwissProt (Human): P25963SwissProt (Mouse) : Q9Z1E3SwissProt (Rat) : Q63746Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of IKB alpha (Phospho-S32/S36) expression in SHSY5Y (A), mouse spleen (B), rat lung (C) whole cell lysates.
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  Immunohistochemical analysis of IKB alpha (Phospho-S32/S36) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of IKB alpha (Phospho-S32/S36) staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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  Direct ELISA antibody dose-response curve using Anti-IKB alpha (Phospho-S32/S36) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.