Description: Rabbit polyclonal antibody to NDUFS1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human NDUFS1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: NDUFS1 Alternative Names: NADH-ubiquinone oxidoreductase 75 kDa subunit mitochondrial; Complex I-75kD; CI-75kDEntrez Gene (Human): 4719Entrez Gene (Mouse) : 227197Entrez Gene (Rat) : 301458SwissProt (Human): P28331SwissProt (Mouse) : Q91VD9SwissProt (Rat) : Q66HF1Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of NDUFS1 expression in mouse kidney (A), mouse heart (B), rat kidney (C), rat heart (D) whole cell lysates.
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Immunohistochemical analysis of NDUFS1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.