Description: Rabbit polyclonal antibody to NDUFA9 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NDUFA9. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: NDUFA9 Alternative Names: NDUFS2L; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9 mitochondrial; Complex I-39kD; CI-39kD; NADH-ubiquinone oxidoreductase 39 kDa subunitEntrez Gene (Human): 4704Entrez Gene (Mouse) : 66108Entrez Gene (Rat) : 362440SwissProt (Human): Q16795SwissProt (Mouse) : Q9DC69SwissProt (Rat) : Q5BK63Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of NDUFA9 expression in Hela (A), A375 (B), mouse heart (C), mouse brain (D), rat heart (E), rat brain (F) whole cell lysates.
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Immunohistochemical analysis of NDUFA9 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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