Anti-MYD88 Antibody-Primary Antibody-Cohesion Biosciences

Western blot analysis of MYD88 expression in HEK293T (A), Hela (B), mouse lung (C), mouse muscle (D), mouse spleen (E), rat muscle (F), rat spleen (G) whole cell lysates.
Immunohistochemical analysis of MYD88 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of MYD88 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Product Name	Anti-MYD88 Antibody
Cat No:	CPA1779
Source:	Rabbit
Reactivity:	H, M, R, B, Mk, P, S
Applications:	WB, IH, IF/IC
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish

Size

Price

200 μl

$350

100 μl

$220

30 μl

$110

Add to cart Ship in 3 days My orders

Description: Rabbit polyclonal antibody to MYD88

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MYD88. The exact sequence is proprietary.

Purification: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/100 - 1/500)

Gene Symbol: MyD88

Alternative Names: Myeloid differentiation primary response protein MyD88

Entrez Gene (Human): 4615

Entrez Gene (Mouse) : 17874

Entrez Gene (Rat) : 301059

SwissProt (Human): Q99836

SwissProt (Mouse) : P22366

SwissProt (Rat) : Q6Y1S1

Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Western blot analysis of MYD88 expression in HEK293T (A), Hela (B), mouse lung (C), mouse muscle (D), mouse spleen (E), rat muscle (F), rat spleen (G) whole cell lysates.
Immunohistochemical analysis of MYD88 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of MYD88 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Sophora flavescens-Angelica sinensis in the treatment of eczema by inhibiting TLR4/MyD88/NF-κB pathway

Journal J Ethnopharmacol

IF 5.4

Application WB

Reactivity Mouse

PMID 38154523

Si Jun Zi decoction inhibits the growth of lung cancer by reducing the expression of PD-L1 through TLR4/MyD88/NF-κB pathway

Journal Journal of Ethnopharmacology

IF 5.4

Application WB

Reactivity Mouse

PMID 37482260

Recently Viewed

Service