Description: Rabbit polyclonal antibody to MMP1 22k Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MMP1 22k. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: MMP1 Alternative Names: CLG; Interstitial collagenase; Fibroblast collagenase; Matrix metalloproteinase-1; MMP-1Entrez Gene (Human): 4312SwissProt (Human): P03956SwissProt (Rat) : P81563Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of MMP1 22k expression in HEK293T (A), Hela (B), Patu8898 (C), A375 (D), mouse kidney (E), rat testis (F), rat kidney (G) whole cell lysates.
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  Immunohistochemical analysis of MMP1 22k staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.