Description: Rabbit polyclonal antibody to FOXO4 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human FOXO4. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: FOXO4 Alternative Names: AFX; AFX1; MLLT7; Forkhead box protein O4; Fork head domain transcription factor AFX1Entrez Gene (Human): 4303Entrez Gene (Mouse) : 54601SwissProt (Human): P98177SwissProt (Mouse) : Q9WVH3Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of FOXO4 expression in HeLa (A), MM142 (B), HuT78 (C), NIH3T3 (D) whole cell lysates.
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Immunohistochemical analysis of FOXO4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Transforming growth factor-beta regulates basal transcriptional regulatory machinery to control cell proliferation and differentiation in cranial neural crest-derived osteoprogenitor cells