Description: Rabbit polyclonal antibody to MCM4 (Phospho-S54) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S54 of human MCM4 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: MCM4 Alternative Names: CDC21; DNA replication licensing factor MCM4; CDC21 homolog; P1-CDC21Entrez Gene (Human): 4173Entrez Gene (Mouse) : 17217SwissProt (Human): P33991SwissProt (Mouse) : P49717Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of MCM4 (Phospho-S54) expression in U2OS (A), rat testis (B), rat heart (C) whole cell lysates.
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  Immunohistochemical analysis of MCM4 (Phospho-S54) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.