Description: Rabbit polyclonal antibody to TAU (Phospho-T534) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T534 of human TAU protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: MAPT Alternative Names: MAPTL; MTBT1; TAU; Microtubule-associated protein tau; Neurofibrillary tangle protein; Paired helical filament-tau; PHF-tauEntrez Gene (Human): 4137Entrez Gene (Mouse) : 17762SwissProt (Human): P10636SwissProt (Mouse) : P10637SwissProt (Rat) : P19332Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of TAU (Phospho-T534) expression in rat brain (A), mouse brain (B) whole cell lysates.
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  Immunohistochemical analysis of TAU (Phospho-T534) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Direct ELISA antibody dose-response curve using Anti-TAU (Phospho-T534) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.