Description: Rabbit polyclonal antibody to MAGEA1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human MAGEA1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: MAGEA1 Alternative Names: MAGE1; MAGE1A; Melanoma-associated antigen 1; Antigen MZ2-E; Cancer/testis antigen 1.1; CT1.1; MAGE-1 antigenEntrez Gene (Human): 4100SwissProt (Human): P43355Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of MAGEA1 expression in U2OS (A), A375 (B) whole cell lysates.
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Immunohistochemical analysis of MAGEA1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.