Description: Rabbit polyclonal antibody to SMAD1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human SMAD1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: SMAD1; SMAD5; SMAD9 Alternative Names: SMAD1; BSP1; MADH1; MADR1; Mothers against decapentaplegic homolog 1; MAD homolog 1; Mothers against DPP homolog 1; JV4-1; Mad-related protein 1; SMAD family member 1; SMAD 1; Smad1; hSMAD1; Transforming growth factor-beta-signaling protein 1; BSP-1; SMAD5; MADH5; Mothers against decapentaplegic homolog 5; MAD homolog 5; Mothers against DPP homolog 5; JV5-1; SMAD family member 5; SMAD 5; Smad5; hSmad5; SMAD9; MADH6; MADH9; Mothers against decapentaplegic homolog 9; MAD homolog 9; Mothers against DPP homolog 9; Madh6; SMAD family member 9; SMAD 9; Smad9Entrez Gene (Human): 4086; 4090; 4093Entrez Gene (Mouse) : 17125; 17129; 55994Entrez Gene (Rat) : 59328; 85435SwissProt (Human): Q15797; Q99717; O15198SwissProt (Mouse) : P70340; P97454; Q9JIW5SwissProt (Rat) : P97588; Q9R1V3; O54835Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of SMAD1 expression in A375 (A), H460 (B) whole cell lysates.
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  Immunohistochemical analysis of SMAD1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.