Description: Rabbit polyclonal antibody to TNF beta Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TNF beta. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: LTA Alternative Names: TNFB; TNFSF1; Lymphotoxin-alpha; LT-alpha; TNF-beta; Tumor necrosis factor ligand superfamily member 1Entrez Gene (Human): 4049SwissProt (Human): P01374Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of TNF beta expression in HEK293T (A), Hela (B), mouse testis (C), rat spleen (D), rat testis (E) whole cell lysates.
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  Immunohistochemical analysis of TNF beta staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.