Description: Rabbit polyclonal antibody to Lamin B1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Lamin B1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: LMNB1 Alternative Names: LMN2; LMNB; Lamin-B1Entrez Gene (Human): 4001Entrez Gene (Mouse) : 16906Entrez Gene (Rat) : 116685SwissProt (Human): P20700SwissProt (Mouse) : P14733SwissProt (Rat) : P70615Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Lamin B1 expression in 293T (A), Hela (B), K562 (C), mouse brain (D), mouse liver (E), rat brain (F), rat liver (G) whole cell lysates. (Predicted band size: 66 kD; Observed band size: 66-70 kD)
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Immunohistochemical analysis of Lamin B1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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