Description: Rabbit polyclonal antibody to Galectin 1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Galectin 1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: LGALS1 Alternative Names: Galectin-1; Gal-1; 14 kDa laminin-binding protein; HLBP14; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; Galaptin; HBL; HPL; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; Putative MAPK-activating protein PM12; S-Lac lectin 1Entrez Gene (Human): 3956Entrez Gene (Mouse) : 16852Entrez Gene (Rat) : 56646SwissProt (Human): P09382SwissProt (Mouse) : P16045SwissProt (Rat) : P11762Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Galectin 1 expression in A549 (A), HL60 (B) whole cell lysates.
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Immunohistochemical analysis of Galectin 1 staining in human kidney cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.