Description: Rabbit polyclonal antibody to Kir4.1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Kir4.1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: KCNJ10 Alternative Names: ATP-sensitive inward rectifier potassium channel 10; ATP-dependent inwardly rectifying potassium channel Kir4.1; Inward rectifier K(+) channel Kir1.2; Potassium channel, inwardly rectifying subfamily J member 10Entrez Gene (Human): 3766Entrez Gene (Mouse) : 16513Entrez Gene (Rat) : 29718SwissProt (Human): P78508SwissProt (Mouse) : Q9JM63SwissProt (Rat) : P49655Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Kir4.1 expression in Hela (A), rat kidney (B) whole cell lysates.
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Immunohistochemical analysis of Kir4.1 staining in human stomach cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.