Description: Rabbit polyclonal antibody to CD104 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CD104. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: ITGB4 Alternative Names: Integrin beta-4; GP150; CD104Entrez Gene (Human): 3691Entrez Gene (Mouse) : 192897Entrez Gene (Rat) : 25724SwissProt (Human): P16144SwissProt (Mouse) : A2A863SwissProt (Rat) : Q64632Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CD104 expression in A431 (A), SW480 (B) whole cell lysates.
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  Immunohistochemical analysis of CD104 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.