Description: Rabbit polyclonal antibody to CD61 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CD61. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: ITGB3 Alternative Names: GP3A; Integrin beta-3; Platelet membrane glycoprotein IIIa; GPIIIa; CD61Entrez Gene (Human): 3690Entrez Gene (Mouse) : 16416SwissProt (Human): P05106SwissProt (Mouse) : O54890Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CD61 expression in HeLa (A), Raw264.7 (B), NIH3T3 (C), H9C2 (D) whole cell lysates.
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Immunohistochemical analysis of CD61 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.