Description: Rabbit polyclonal antibody to CD49c Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD49c. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: ITGA3 Alternative Names: MSK18; Integrin alpha-3; CD49 antigen-like family member C; FRP-2; Galactoprotein B3; GAPB3; VLA-3 subunit alpha; CD49cEntrez Gene (Human): 3675Entrez Gene (Mouse) : 16400SwissProt (Human): P26006SwissProt (Mouse) : Q62470Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CD49c expression in HepG2 (A), AML12 (B), PC12 (C), A549 (D) whole cell lysates.
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Immunohistochemical analysis of CD49c staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.