Description: Rabbit polyclonal antibody to IRS1 (Phospho-S307) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S307 of human IRS1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: IRS1 Alternative Names: Insulin receptor substrate 1; IRS-1Entrez Gene (Human): 3667Entrez Gene (Mouse) : 16367Entrez Gene (Rat) : 25467SwissProt (Human): P35568SwissProt (Mouse) : P35569SwissProt (Rat) : P35570Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of IRS1 (Phospho-S307) expression in HEK293T insulin-treated (A) whole cell lysates.
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  Immunohistochemical analysis of IRS1 (Phospho-S307) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.