Description: Rabbit polyclonal antibody to IRAK1 (Phospho-T209) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T209 of human IRAK1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: IRAK1 Alternative Names: IRAK; Interleukin-1 receptor-associated kinase 1; IRAK-1Entrez Gene (Human): 3654Entrez Gene (Mouse) : 16179SwissProt (Human): P51617SwissProt (Mouse) : Q62406Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of IRAK1 (Phospho-T209) expression in A2780 (A), rat thymus (B) whole cell lysates.
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Immunohistochemical analysis of IRAK1 (Phospho-T209) staining in human kidney cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.