Description: Rabbit polyclonal antibody to IL-5 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IL-5. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: IL5 Alternative Names: Interleukin-5; IL-5; B-cell differentiation factor I; Eosinophil differentiation factor; T-cell replacing factor; TRFEntrez Gene (Human): 3567Entrez Gene (Mouse) : 16191SwissProt (Human): P05113SwissProt (Mouse) : P04401SwissProt (Rat) : Q08125Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of IL-5 expression in HEK293T (A), K562 (B), mouse liver (C), rat testis (D), rat liver (E) whole cell lysates.
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  Immunohistochemical analysis of IL-5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.