Description: Rabbit polyclonal antibody to Factor I Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Factor I. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: CFI Alternative Names: IF; Complement factor I; C3B/C4B inactivatorEntrez Gene (Human): 3426Entrez Gene (Mouse) : 12630Entrez Gene (Rat) : 79126SwissProt (Human): P05156SwissProt (Mouse) : Q61129SwissProt (Rat) : Q9WUW3Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Factor I expression in A549 (A), mouse kidney (B), rat liver (C), rat kidney (D) whole cell lysates.
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Immunohistochemical analysis of Factor I staining in human kidney cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.