Description: Rabbit polyclonal antibody to CD54 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CD54. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: ICAM1 Alternative Names: Intercellular adhesion molecule 1; ICAM-1; Major group rhinovirus receptor; CD54Entrez Gene (Human): 3383Entrez Gene (Mouse) : 15894SwissProt (Human): P05362SwissProt (Mouse) : P13597Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CD54 expression in A549 (A), HepG2 (B), AML12 (C) whole cell lysates.
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  Immunohistochemical analysis of CD54 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.