Description: Rabbit polyclonal antibody to 5-HT2A Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human 5-HT2A. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: HTR2A Alternative Names: HTR2; 5-hydroxytryptamine receptor 2A; 5-HT-2; 5-HT-2A; Serotonin receptor 2AEntrez Gene (Human): 3356Entrez Gene (Mouse) : 15558Entrez Gene (Rat) : 29595SwissProt (Human): P28223SwissProt (Mouse) : P35363SwissProt (Rat) : P14842Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of 5-HT2A expression in mouse kidney (A), rat kidney (B) whole cell lysates.
-
Immunohistochemical analysis of 5-HT2A staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.