Description: Rabbit polyclonal antibody to GRP75 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GRP75. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500) Gene Symbol: HSPA9 Alternative Names: HSPA9; GRP75; HSPA9B; Stress-70 protein mitochondrial; 75 kDa glucose-regulated protein; GRP-75; Heat shock 70 kDa protein 9; Mortalin; MOT; Peptide-binding protein 74; PBP74Entrez Gene (Human): 3313Entrez Gene (Mouse) : 15526Entrez Gene (Rat) : 291671SwissProt (Human): P38646; Q8N1C8SwissProt (Mouse) : P38647SwissProt (Rat) : P48721Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of GRP75 expression in HEK293T (A), Jurkat (B), MCF7 (C), mouse brain (D), mouse liver (E), rat brain (F), rat liver (G) whole cell lysates.
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  Immunohistochemical analysis of GRP75 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of GRP75 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.