Description: Rabbit polyclonal antibody to NRAS/HRAS/KRAS Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human NRAS/HRAS/KRAS. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/200) Gene Symbol: NRAS; HRAS; KRAS Alternative Names: NRAS; HRAS1; GTPase NRas; Transforming protein N-Ras; HRAS; HRAS1; GTPase HRas; H-Ras-1; Ha-Ras; Transforming protein p21; c-H-ras; p21ras; KRAS; KRAS2; RASK2; GTPase KRas; K-Ras 2; Ki-Ras; c-K-ras; c-Ki-rasEntrez Gene (Human): 3265; 3845; 4893Entrez Gene (Mouse) : 15461; 16653Entrez Gene (Rat) : 24605; 293621; 24525SwissProt (Human): P01111; P01112; P01116SwissProt (Mouse) : P08556; Q61411; P32883SwissProt (Rat) : Q04970; P20171; P08644Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of NRAS/HRAS/KRAS expression in H446 (A), mouse kidney (B), mouse testis (C), rat kidney (D) whole cell lysates.
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  Immunohistochemical analysis of NRAS/HRAS/KRAS staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of NRAS/HRAS/KRAS staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).