Description: Rabbit polyclonal antibody to NUR77 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NUR77. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: NR4A1 Alternative Names: GFRP1; HMR; NAK1; Nuclear receptor subfamily 4 group A member 1; Early response protein NAK1; Nuclear hormone receptor NUR/77; Nur77; Orphan nuclear receptor HMR; Orphan nuclear receptor TR3; ST-59; Testicular receptor 3Entrez Gene (Human): 3164Entrez Gene (Mouse) : 15370Entrez Gene (Rat) : 79240SwissProt (Human): P22736SwissProt (Mouse) : P12813SwissProt (Rat) : P22829Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of NUR77 expression in rat muscle (A) whole cell lysates.
-
Immunohistochemical analysis of NUR77 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.