Description: Rabbit polyclonal antibody to HLA-DOB Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human HLA-DOB. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: HLA-DOB Alternative Names: HLA class II histocompatibility antigen, DO beta chain; MHC class II antigen DOBEntrez Gene (Human): 3112SwissProt (Human): P13765Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of HLA-DOB expression in mouse lung (A), rat lung (B) whole cell lysates.
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  Immunohistochemical analysis of HLA-DOB staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.