Description: Rabbit polyclonal antibody to mGLUR8 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human mGLUR8. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: GRM8 Alternative Names: GPRC1H; MGLUR8; Metabotropic glutamate receptor 8; mGluR8Entrez Gene (Human): 2918Entrez Gene (Mouse) : 14823Entrez Gene (Rat) : 60590SwissProt (Human): O00222SwissProt (Mouse) : P47743SwissProt (Rat) : P70579Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of mGLUR8 expression in HCT116 (A), HepG2 (B), Hela (C), AML12 (D) whole cell lysates.
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Immunohistochemical analysis of mGLUR8 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.