Description: Rabbit polyclonal antibody to GRB14 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GRB14. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: GRB14 Alternative Names: Growth factor receptor-bound protein 14; GRB14 adapter proteinEntrez Gene (Human): 2888SwissProt (Human): Q14449Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GRB14 expression in MOLT4 (A), COLO205 (B) whole cell lysates.
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Immunohistochemical analysis of GRB14 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Grb14 as an independent good prognosis factor for breast cancer patients treated with neoadjuvant chemotherapy