Description: Rabbit polyclonal antibody to GRK5 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GRK5. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: GRK5 Alternative Names: GPRK5; G protein-coupled receptor kinase 5; G protein-coupled receptor kinase GRK5Entrez Gene (Human): 2869Entrez Gene (Mouse) : 14773Entrez Gene (Rat) : 59075SwissProt (Human): P34947SwissProt (Mouse) : Q8VEB1SwissProt (Rat) : Q62833Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of GRK5 expression in Hela (A), H446 (B), mouse kidney (C), mouse lung (D), rat kidney (E) whole cell lysates.
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  Immunohistochemical analysis of GRK5 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.