Description: Rabbit polyclonal antibody to GPR18 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GPR18. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), FC (1/100 - 1/400) Gene Symbol: GPR18 Alternative Names: GPCRW; N-arachidonyl glycine receptor; NAGly receptor; G-protein coupled receptor 18Entrez Gene (Human): 2841Entrez Gene (Mouse) : 110168Entrez Gene (Rat) : 679957SwissProt (Human): Q14330SwissProt (Mouse) : Q8K1Z6SwissProt (Rat) : A1A5S3Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of GPR18 expression in Jurkat (A) whole cell lysates.
• 
  Immunofluorescent analysis of GPR18 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).