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Anti-Connexin 32 Antibody CPA1472
  • Western blot analysis of Connexin 32 expression in LOVO (A), mouse brain (B), mouse lung (C), mouse kidney (D), rat kidney (E) whole cell lysates.
  • Immunohistochemical analysis of Connexin 32 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. AEC was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product NameAnti-Connexin 32 Antibody
Cat No: CPA1472
Source: Rabbit
Reactivity: H, M, R, B, D, P, Mk
Applications: WB, IH
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
200 μl
$350
100 μl
$220
30 μl
$110
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Description: Rabbit polyclonal antibody to Connexin 32
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Connexin 32. The exact sequence is proprietary.
Purification: The antibody was purified by immunogen affinity chromatography.
Clonality: Polyclonal
Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)
Gene Symbol: GJB1
Alternative Names: CX32; Gap junction beta-1 protein; Connexin-32; Cx32; GAP junction 28 kDa liver protein
Entrez Gene (Human): 2705
Entrez Gene (Mouse) : 14618
Entrez Gene (Rat) : 29584
SwissProt (Human): P08034
SwissProt (Mouse) : P28230
SwissProt (Rat) : P08033
Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of Connexin 32 expression in LOVO (A), mouse brain (B), mouse lung (C), mouse kidney (D), rat kidney (E) whole cell lysates.
  • Immunohistochemical analysis of Connexin 32 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. AEC was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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