Anti-GHRH Receptor Antibody-Primary Antibody-Cohesion Biosciences

Western blot analysis of GHRH Receptor expression in mouse kidney (A), rat kidney (B) whole cell lysates.
Immunofluorescent analysis of GHRH Receptor staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Product Name	Anti-GHRH Receptor Antibody
Cat No:	CPA1470
Source:	Rabbit
Reactivity:	H, M, R, Mk
Applications:	WB, IF/IC
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish

Size

Price

200 μl

$350

100 μl

$220

30 μl

$110

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Datasheet

MSDS

Description: Rabbit polyclonal antibody to GHRH Receptor

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GHRH Receptor. The exact sequence is proprietary.

Purification: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)

Gene Symbol: GHRHR

Alternative Names: Growth hormone-releasing hormone receptor; GHRH receptor; Growth hormone-releasing factor receptor; GRF receptor; GRFR

Entrez Gene (Human): 2692

Entrez Gene (Mouse) : 14602

Entrez Gene (Rat) : 25321

SwissProt (Human): Q02643

SwissProt (Mouse) : P32082

SwissProt (Rat) : Q02644

Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Western blot analysis of GHRH Receptor expression in mouse kidney (A), rat kidney (B) whole cell lysates.
Immunofluorescent analysis of GHRH Receptor staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Stabilization of superoxide dismutase by acetyl-l-carnitine in human brain endothelium during alcohol exposure: Novel protective approach

Journal Free Radic Biol Med

IF 7.4

Application ICC/IF

Reactivity Mouse

PMID 21782933

Transgenic mice expressing the human growth hormone gene provide a model system to study human growth hormone synthesis and secretion in non-tumor-derived pituitary cells: differential effects of dexamethasone and thyroid hormone

Journal Mol Cell Endocrinol

IF 4.1

Application ICC/IF

Reactivity Mouse

PMID 21777655

Agonist of growth hormone-releasing hormone as a potential effector for survival and proliferation of pancreatic islets

Journal Proc Natl Acad Sci U S A

Application WB, IHC-P

Reactivity Rat

PMID 20616039

Cardioprotective effects of growth hormone-releasing hormone agonist after myocardial infarction

Journal Proc Natl Acad Sci U S A

Application WB

Reactivity Rat

PMID 20133784

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