Description: Rabbit polyclonal antibody to CD174 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD174. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: FUT3 Alternative Names: FT3B; LE; Galactoside 3(4)-L-fucosyltransferase; Blood group Lewis alpha-4-fucosyltransferase; Lewis FT; Fucosyltransferase 3; Fucosyltransferase III; FucT-IIIEntrez Gene (Human): 2525SwissProt (Human): P21217Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CD174 expression in H1792 (A), H1688 (B), mouse testis (C), mouse kidney (D), rat testis (E) whole cell lysates.
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Immunohistochemical analysis of CD174 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.