Description: Rabbit polyclonal antibody to FOXO1 (Phospho-S319) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S319 of human FOXO1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500) Gene Symbol: FOXO1 Alternative Names: FKHR; FOXO1A; Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcomaEntrez Gene (Human): 2308Entrez Gene (Mouse) : 56458Entrez Gene (Rat) : 84482SwissProt (Human): Q12778SwissProt (Mouse) : Q9R1E0SwissProt (Rat) : G3V7R4Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of FOXO1 (Phospho-S319) expression in Jurkat (A), MCF7 (B), mouse testis (C) whole cell lysates.
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  Immunohistochemical analysis of FOXO1 (Phospho-S319) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of FOXO1 (Phospho-S319) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.