Description: Rabbit polyclonal antibody to FHL2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human FHL2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: FHL2 Alternative Names: DRAL; SLIM3; Four and a half LIM domains protein 2; FHL-2; LIM domain protein DRAL; Skeletal muscle LIM-protein 3; SLIM-3Entrez Gene (Human): 2274Entrez Gene (Mouse) : 14200Entrez Gene (Rat) : 63839SwissProt (Human): Q14192SwissProt (Mouse) : O70433SwissProt (Rat) : O35115Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of FHL2 expression in HEK293T (A), mouse muscle (B), rat heart (C), rat muscle (D) whole cell lysates.
-
Immunofluorescent analysis of FHL2 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).