Description: Rabbit polyclonal antibody to Factor VIII Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Factor VIII. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: F8 Alternative Names: F8C; Coagulation factor VIII; Antihemophilic factor; AHF; Procoagulant componentEntrez Gene (Human): 2157SwissProt (Human): P00451Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Factor VIII expression in HeLa (A), HepG2 (B) whole cell lysates.
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Immunohistochemical analysis of Factor VIII staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.