Description: Rabbit polyclonal antibody to ERCC1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ERCC1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: ERCC1 Alternative Names: DNA excision repair protein ERCC-1Entrez Gene (Human): 2067Entrez Gene (Mouse) : 13870SwissProt (Human): P07992SwissProt (Mouse) : P07903Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ERCC1 expression in MCF7 (A), HeLa (B), A431 (C), mouse liver (D), rat kidney (E) whole cell lysates.
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  Immunohistochemical analysis of ERCC1 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.